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. Author manuscript; available in PMC: 2015 Jun 26.
Published in final edited form as: Breast Cancer Res Treat. 2012 Oct 9;136(2):443–455. doi: 10.1007/s10549-012-2282-3

Figure 10. Soft agar growth assay of MCF- and MCF-7/TAM Res cells.

Figure 10

MCF-7 and MCF-7/Tamoxifen resistant cells (MCF-7TR1) were seeded (5,000/well) in 0.35% agarose and then treated with vehicle (C), E2 (10nM), bergapten (B50μM). Cells were allowed to grow for 14 days and the number of colonies ≥ 50μm were quantified and the results were graphed. ** P< 0.01 versus untreated cells; ° P< 0.05 ; °° P< 0.01 versus E2 condition.