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. Author manuscript; available in PMC: 2015 Jun 26.
Published in final edited form as: Breast Cancer Res Treat. 2012 Oct 9;136(2):443–455. doi: 10.1007/s10549-012-2282-3

Figure 6. Immunoblot of ERα and SMAD4/SMAD3 in breast cancer cells.

Figure 6

(A) The protein lysates of MCF-7 and MCF-7/Tam Res cells treated for 24h with different concentrations of bergapten (B) as indicated, were immunoblotted with anti-ERα, anti-SMAD3 and anti-SMAD4. GAPDH was used as loading control. MCF-7/Tam Res cells were maintained (+Tam) or not (−Tam) with OH-Tamoxifen (T1μM) during the experimental procedure IgG: negative control of Immunoprecipitation experiment. Results are representative of three independent experiments.

(B) Optical density of the ERα and SMAD4/SMAD3 ratio.