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. 2015 Jun 26;14:92. doi: 10.1186/s12934-015-0282-9

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© Chen et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Expression and secretion of α-amylase (AmyL and AmyS) in B. subtilis 1A751. A Vector map of E. coli/B. subtilis shuttle plasmid pMA5. P_HpaII, a widely used promoter from Staphylococcus aureus; RBS, ribosome binding site; ColE1, origin of replication for E. coli; bla, ampicillin resistance; RepB, origin of replication for B. subtilis; neo, kanamycin resistance. B The gene fragments of α-amylase (AmyL and AmyS). Fragment a: AmyL encoding gene containing its native signal peptide SP_amyl plus T7 terminator; fragment b: AmyS encoding gene containing its native signal peptide SP_amys plus T7 terminator. C Starch hydrolysis of B. subtilis 1A751 (1), 1A751 (pMA5) (2), 1A751 (pMA5L) (3), 1A751 (pMA5S) (4). Strains were grown on LB agar medium containing 1% starch at 37°C overnight. Flood the surface of the plate with 5 mL Gram’s iodine stain. D SDS-PAGE analysis of expression of α-amylase (AmyL and AmyS) in medium and cell fractions by B. subtilis at incubation of 72 h. Lane M molecular weight marker.