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. 2015 Jun 26;14:92. doi: 10.1186/s12934-015-0282-9

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© Chen et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Optimization of PrsA overexpression level in B. subtilis. 1A23 (pMA5L) and 1A23 (pMA5S) were grown in SR medium supplemented with xylose of different concentrations [0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 4.5 and 5.0 % (w/v)]. a Analysis of α-amylase (AmyL and AmyS) activity in the supernatant at incubation of 72 h. The α-amylase activity was converted to percent increase referring to 2.0% xylose. b SDS-PAGE analysis of α-amylase (AmyL and AmyS) distribution in culture supernatants.