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. 2015 Jun 26;14:92. doi: 10.1186/s12934-015-0282-9

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© Chen et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Overexpression of PrsA combined with the screened components in B. subtilis. a The transcriptional levels of the genes or gene operons in B. subtilis. The first figure shows the transcriptional level of prsA in recombinant strains; the transcription level of 1A751 (control) is regarded as 100%. The second figure indicates the transcriptional levels of nine genes or gene operons in nine corresponding strains, respectively; Control, the transcriptional levels of nine genes or gene operons (all regarded as 100%) in 1A751. b Analysis of α-amylase (AmyL and AmyS) activity in nine combinational overexpression strains. The α-amylase activity was converted to percent increase referring to the respective control strains [the former, 1A23 (pMA5L); the latter, 1A23 (pMA5S)]. c SDS-PAGE analysis of α-amylase (AmyL and AmyS) in nine combinational overexpression strains. Lane M molecular weight marker. The former Control is 1A23 (pMA5L); the latter Control is 1A23 (pMA5S). Specifically, the genes or gene operons SRP, ftsy, secA, secYEG, secDF, groESL operon, partial dnaK operon, sipT and sipS in a, b and c corresponded to strains 1A231, 1A232, 1A233, 1A234, 1A235, 1A236, 1A237, 1A238 and 1A239, respectively.