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. 2015 Jun 26;14:92. doi: 10.1186/s12934-015-0282-9

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© Chen et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Characterization of engineered and parental strains. B. subtilis 1A751 (pMA5L) (parental strain), 1A237 (pMA5L) (engineered strain), 1A751 (pMA5S) (parental strain) and 1A237 (pMA5S) (engineered strain) were cultivated for 96 h at 37°C in 30 mL SR medium supplemented with 4% xylose (w/v). a Growth of the strains. b Analysis of α-amylase (AmyL and AmyS) activity. c Analysis of productivity. d SDS-PAGE analysis of α-amylase (AmyL and AmyS) at 72 h.