Table 1.
Strains used in this study
| Strains | Genotype and/or relevant characteristic(s) | Source |
|---|---|---|
| E. coli DH5α | F−∆lacU169(Ø80d lacZ∆M15) supE44 hsdR17 recA1 gyrA96 endA1 thi-1 relA1 | Invitrogen |
| B. licheniformis CICC 10181 | Wild-type B. licheniformis, amyl gene | CICC |
| G. stearothermophilus ATCC 31195 | Wild-type G. stearothermophilus, amys gene | ATCC |
| B. subtilis 1A751 | eglS∆102 bglT/bglS∆EV aprE nprE his | BGSC |
| 1AXBG | 1A751 with integration of pDDXBG (amyE::PxylA-bgaB); Cmr | This work |
| 1AXGB | 1A751 with integration of pDDXGB (amyE::Pgrac-bgaB); Cmr | This work |
| 1A01 | 1A751 with integration of pDD01 (amyE::PxylA-ffh); Cmr | This work |
| 1A02 | 1A751 with integration of pDD02 (amyE::PxylA-hbs); Cmr | This work |
| 1A03 | 1A751 with integration of pDD03 (amyE::PxylA-scr); Cmr | This work |
| 1A04 | 1A751 with integration of pDD04 (amyE::PxylA-SRP); Cmr | This work |
| 1A05 | 1A751 with integration of pDD05 (amyE::PxylA-ftsy); Cmr | This work |
| 1A06 | 1A751 with integration of pDD06 (amyE::PxylA-csaA); Cmr | This work |
| 1A07 | 1A751 with integration of pDD07 (amyE::PxylA-secA); Cmr | This work |
| 1A08 | 1A751 with integration of pDD08 (amyE::PxylA-secY); Cmr | This work |
| 1A09 | 1A751 with integration of pDD09 (amyE::PxylA-secE); Cmr | This work |
| 1A10 | 1A751 with integration of pDD10 (amyE::PxylA-secG); Cmr | This work |
| 1A11 | 1A751 with integration of pDD11 (amyE::PxylA-secYEG); Cmr | This work |
| 1A12 | 1A751 with integration of pDD12 (amyE::PxylA-secDF); Cmr | This work |
| 1A13 | 1A751 with integration of pDD13 (amyE::PxylA-yrbF); Cmr | This work |
| 1A14 | 1A751 with integration of pDD14 (amyE::PxylA-spoIIIJ); Cmr | This work |
| 1A15 | 1A751 with integration of pDD15 (amyE::PxylA-yqjG); Cmr | This work |
| 1A16 | 1A751 with integration of pDD16 (amyE::PxylA-sipT); Cmr | This work |
| 1A17 | 1A751 with integration of pDD17 (amyE::PxylA-sipS); Cmr | This work |
| 1A18 | 1A751 with integration of pDD18 (amyE::PxylA-sipU); Cmr | This work |
| 1A19 | 1A751 with integration of pDD19 (amyE::PxylA-sipV); Cmr | This work |
| 1A20 | 1A751 with integration of pDD20 (amyE::PxylA-sipW); Cmr | This work |
| 1A21 | 1A751 with integration of pDD21 (amyE::PxylA-groESL operon); Cmr | This work |
| 1A22 | 1A751 with integration of pDD22 (amyE::PxylA-partial dnaK operon); Cmr | This work |
| 1A23 | 1A751 with integration of pDD23 (amyE::PxylA-prsA); Cmr | This work |
| 1A231 | 1A751 with integration of pDD231 (amyE::PxylA-prsA, Pgrac-SRP); Cmr | This work |
| 1A232 | 1A751 with integration of pDD232 (amyE::PxylA-prsA, Pgrac-ftsy); Cmr | This work |
| 1A233 | 1A751 with integration of pDD233 (amyE::PxylA-prsA, Pgrac-secA); Cmr | This work |
| 1A234 | 1A751 with integration of pDD234 (amyE::PxylA-prsA, Pgrac-secYEG); Cmr | This work |
| 1A235 | 1A751 with integration of pDD235 (amyE::PxylA-prsA, Pgrac-secDF); Cmr | This work |
| 1A236 | 1A751 with integration of pDD236 (amyE::PxylA-prsA, Pgrac-groESL operon); Cmr | This work |
| 1A237 | 1A751 with integration of pDD237 (amyE::PxylA-prsA, Pgrac-partial dnaK opeon); Cmr | This work |
| 1A238 | 1A751 with integration of pDD238 (amyE::PxylA-prsA, Pgrac-sipT); Cmr | This work |
| 1A239 | 1A751 with integration of pDD239 (amyE::PxylA-prsA, Pgrac-sipS); Cmr | This work |
CICC China Center of Industrial Culture Collection (http://www.chinacicc.org), ATCC American Type Culture Collection, BGSC Bacillus Genetic Stock Center, USA.