Table 2.
Plasmids used in this study
| Plasmids | Genotype and/or relevant characteristic(s) | Source |
|---|---|---|
| pET28-a(+) | E. coli expression plasmid, T7 promoter, T7 terminator, Kanr | Lab stock |
| pET28L | pET28-a(+) derivative, amyl | This work |
| pET28S | pET28-a(+) derivative, amys | This work |
| pMA5 | E. coli/B. subtilis shuttle vector, PHpaII, Apr, Kmr | BGSC |
| pMA5L | pMA5 derivative, SPamyl, amyl, T4 terminator | This work |
| pMA5S | pMA5 derivative, SPamys, amys, T4 terminator | This work |
| pHCMC04 | PxylA, Apr, Cmr | Lab stock |
| pHT43 | Pgrac, SPamyQ, Apr, Cmr | Lab stock |
| pDD | Integration vector, pDL derivative, Apr, Cmr | This work |
| pDDX | pDD derivative, PxylA | This work |
| pDDXG | pDDX derivative, PxylA, Pgrac | This work |
| pDDXBG | pDDXG derivative, bgaB | This work |
| pDDXGB | pDDXG derivative, bgaB | This work |
| pDD01 | pDDXG derivative, ffh | This work |
| pDD02 | pDDXG derivative, hbs | This work |
| pDD03 | pDDXG derivative, scr | This work |
| pDD04 | pDDXG derivative, SRP | This work |
| pDD05 | pDDXG derivative, ftsy | This work |
| pDD06 | pDDXG derivative, csaA | This work |
| pDD07 | pDDXG derivative, secA | This work |
| pDD08 | pDDXG derivative, secY | This work |
| pDD09 | pDDXG derivative, secE | This work |
| pDD10 | pDDXG derivative, secG | This work |
| pDD11 | pDDXG derivative, secYEG | This work |
| pDD12 | pDDXG derivative, secDF | This work |
| pDD13 | pDDXG derivative, yrbF | This work |
| pDD14 | pDDXG derivative, spoIIIJ | This work |
| pDD15 | pDDXG derivative, yqjG | This work |
| pDD16 | pDDXG derivative, sipT | This work |
| pDD17 | pDDXG derivative, sipS | This work |
| pDD18 | pDDXG derivative, sipU | This work |
| pDD19 | pDDXG derivative, sipV | This work |
| pDD20 | pDDXG derivative, sipW | This work |
| pDD21 | pDDXG derivative, groESL operon | This work |
| pDD22 | pDDXG derivative, partial dnaK operon | This work |
| pDD23 | pDDXG derivative, prsA | This work |
| pDD231 | pDD23 derivative, prsA, SRP | This work |
| pDD232 | pDD23 derivative, prsA, ftsy | This work |
| pDD233 | pDD23 derivative, prsA, secA | This work |
| pDD234 | pDD23 derivative, prsA, secYEG | This work |
| pDD235 | pDD23 derivative, prsA, secDF | This work |
| pDD236 | pDD23 derivative, prsA, groESL operon | This work |
| pDD237 | pDD23 derivative, prsA, partial dnaK operon | This work |
| pDD238 | pDD23 derivative, prsA, sipT | This work |
| pDD239 | pDD23 derivative, prsA, sipS | This work |