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. 2015 Jun 1;65(3):208–215. doi: 10.1270/jsbbs.65.208

Fig. 2.

Fig. 2

Validation of S allele-specific primers. PCR fragments were amplified from 10 S allele homozygotes and two accessions with the Sk allele by using the designed primer pairs. M: 100 bp DNA ladder (Toyobo); 1: Im-3 (S1/S1); 2: 312-9 (S2/S2); 3: 312-6 (S3/S3); 4: 421-6 (S4/S4); 5: 421-24 (S5/S5); 6: Im-18 (S6/S6); 7: 420-50 (S7/S7); 8: 548-1 (S8/S8); 9: S9-homozygote (S9/S9); 10: ‘Kinchaku’ (Sk/S4); 11: 314-32 (Sk/S3); 12: ‘Chukanbohon Nashi nou 1 gou’ (S4sm/S4sm).