Table 2.
Characteristics of S allele-specific primer pairs
| S allele | Primer pair | Sequence (5′-3′) | Size of PCR product (bp) |
|---|---|---|---|
| S1 | PpS1 | F: AATGTAAGACTACAGCCCTG R: TCCACCAGTGGCCTGTTTG |
441 |
| S2 | PpS2 | F: TCCTTCCATCAAATTCTCCCCAGCA R: GGGGTACACCGTGCGTCCAT |
731 |
| S3 | PpS3 | F: TGCCCGATAAAGAATATTCG R: CTCTGGTATGCACAAGAGAG |
209 |
| S4 | PpS4 | F: TCTGGGAAAGAGAGTGGCTC R: GGCAATTTATGAACTTAGTC |
309 |
| S5 | PpS5 | F: TTGTGGCCCTCAAGCATGGC R: CGTGCATGAAAATCTATGTTTGAGGAC |
174 |
| S6 | PpS6 | F: GTTTGTGGCCTTCAAACGACG R: GTGATCCTTTAAAAGAACTGC |
621 |
| S7 | PpS7 | F: TCACCCAGAAAATTGCACTAATGC R: CCAGTGGCCTTTGTATTCCCAA |
433 |
| S8 | PpS8 | F: GTCATTGACGGGGTTTGAACCC R: CCAACTGGGCTTTGAGTGAT |
218 |
| S9 | PpS9 | F: CAAAAATGTACCCATGTTTGGT R: CGCCTTTGAGAGGATTTCAG |
702 |
| Sk | PpSk | F: GAAAACCAAGGTGCCTCAGGC R: CTCAACCAATTCAATAGTCCC |
1167 |
| S4sm | SM* | F: TCGTCTTAGGGATTTCCAATGC R: GCCTTAAGGGTTCATTGGGC |
666 |