Fig 5. Inhibition of PI3K blocks the activation of mTOR and Cdk2 induced by TNFα and RANKL.

Pkd1 wild type MEK cells were pretreated with PI3K inhibitor, LY294002 (20 μM, mark as LY), for 1 hour, and then stimulated by TNFα (20 ng/ml) (A) or RANKL (100 ng/ml) (B) for 30 minutes. The expression of phospho-Akt, Akt, phospho-S6 and S6, phospho-Cdk2 and Cdk2 from whole cell lysates was analyzed by western blot. The numbers at the bottom indicate the intensities of p-Akt relative to total Akt, p-S6 relative to total S6 and p-Cdk2 relative to total Cdk2.