Male 8-week–old WT and Sam68-KO mice were firstly matched with body weight and then fed with HFD for 12 weeks, followed by assessments of body compositions, GTT, ITT, and insulin signaling in adipose tissues. (A) Serial measurements of body weight (n=6/group, *p<0.05, **p<0.01). (B) Body compositions by NMR (left panel, fat mass; right panel, lean mass. n=6/group, **p < 0.01, N.S., Not significant). (C) GTT and (D) ITT (n=6, *p<0.05). (E) Western blotting analyses of WAT fat depots from WT and Sam68-KO mice 30 min after i.p. injection of insulin.