Figure 1. Resveratrol induces Egr-1 expression.

A. A549 cells were treated with 100 μM of resveratrol for indicated hours and Egr-1 mRNA levels were measured by real-time RT-PCR. The Egr-1 mRNA expression was adjusted to GAPDH (housekeeping gene) and normalized to untreated control. B. and C. Egr-1 and β-actin (loading control) protein expression was determined by western blotting after indicated resveratrol treatment. D. Fluorescent In Situ Hybridization assay was used to detect Egr-1 mRNA level in non-treated and resveratrol-treated cells. Images were taken on Axio Imager M2 at 400X magnification.