Figure 4. Resveratrol is not sufficient to activate GADD45α.
A. and B. GADD45α protein expression and mRNA level were measured by western blotting and real-time RT PCR after cells were treated with 100 μM of resveratrol for various hours. C. Cells were treated with or without resveratrol overnight and performed anti-GADD45α immunofluorescent staining. Images were taken on Axio Imager M2 at 400X magnification.