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. 2015 May;6(5-6):220–230. doi: 10.18632/genesandcancer.62

Figure 5. Both synthetic and natural promoters are able to overexpress GADD45α to induce cell apoptosis when combined with resveratrol.

Figure 5

A. Cells were co-transfected with luciferase constructs containing either the synthetic CArG promoter (pE5, pE6 and pE9NS) or the natural Egr-1 promoter (pE460) and Renilla luciferase control reporter vector (pRL-TK) followed by resveratrol or non-treatment. Relative luminescence was plotted as the average of triplicate experiment and represented as mean ± SE. B. Level of GADD45α protein was detected by western blotting after cells were transfected with either suicide gene therapy vector (pE460.G45α or pE9NS.G45α) or control vector (pT.Luc) and then treated with or without 100 μM of resveratrol for 24 h. C. MTT assay was performed for cells pre-transfected with pE460.G45α, pE9NS.G45α or pT.Luc and treated with different doses of resveratrol for 24 h. D. Cells were exposed to 100 μM of resveratrol for 24 h after transfected with control or suicide gene therapy constructs. Apoptosis was determined by FACS of Annexin V-FITC and PI stained cells.

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