Fig. 11.

Metabolomic and transcriptomic evidence that transition-stage Gh fiber experiences more oxidative stress than Gb fiber. a) The ratio of reduced glutathione (GSH) to oxidized glutathione (GSSG) in Gb (grey bars) and Gh (white bars) fiber. The ratio is based on the scaled imputed mean for both metabolites. b) The expression level (RPKM) of a putative alternative oxidase (AOX) gene in Gh (white bars) and Gb (grey bars) fiber as derived from RNA-Seq data. The homologous protein in Arabidopsis (AOX2, At5G64210) attenuates ROS production during respiration [91], and the related Gr transcript is Gorai.005G220500.1. Asterisks indicate significant differences between genotypes at a given DPA. Error bars are standard deviation