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. Author manuscript; available in PMC: 2015 Jun 26.
Published in final edited form as: Biofabrication. 2012 Jul 31;4(3):035002. doi: 10.1088/1758-5082/4/3/035002

Figure 1.

Figure 1

EC cord formation is dependent on the percentage of ECs in sequential preculture (phase contrast). Sequential preculture containing 8% ECs (sequential preculture 8%) was performed to allow for capillary cord formation by ECs. The enriched CM fraction was kept constant at the percentage determined most beneficial for regular pre-culture in previous studies (60% enriched CM) and the EC seeding percentage was varied from 8% to 31%. (A) Phase contrast microscopy at 24 h following EC seeding. Sequential preculture 8% group displayed evidence of cords (white arrows) while the other groups showed EC proliferation into flat cell layers. (B) Phase contrast, microscopy after seven days of cultivation. Cardiac FB and enriched CM were added at 24 and 48 h following EC seeding, respectively, and the organoids were cultivated for seven additional days. Sequential preculture 8% formed cylindrical organoids (white arrows) with overall elongated cell morphology, while the other groups remained as two-dimensional cell layers consisting of rounded cells. Enriched CMs also appeared mostly rounded. n = 3–5 samples per group.