Table 1. Protein content of S. suis P1/7 membrane vesicles identified by ES MS/MS in order of abundance in each functional group.

Protein accession number	Gene accession number	MS/MS identification	Molecular weight	Protein name/function	Predicted localization
Translational, ribosomal structure, and biogenesis (n = 3)
C5VVU1	SSU0721	Putative 30S ribosomal protein S1	44 kDa	30S Ribosomal protein S1	Cytoplasm
C5VWE9	SSU1935	30S ribosomal protein S4	23 kDa	30S Ribosomal protein S4	Cytoplasm
C5VVK7	SSU1771	30S ribosomal protein S2	29 kDa	30S Ribosomal protein S2	Cytoplasm
Transport, uptake, and osmoregulation (n = 9)
C5VWT7	SSU0934	Putative lipoprotein	36 kDa	Putative ABC transporter	Membrane
C5W096	SSU1664	Putative oligopeptide-binding protein OppA	66 kDa	OppA / Peptide binding	Unknown
C5W029	SSU0503	Putative amino acid ABC transporter	31 kDa	ABC transporter	Unknown
C5VW03	SSU1853	Putative amino-acid ABC transporter	29 kDa	ABC transporter	Periplasm
C5VXJ7	SSU1170	Extracellular solute-binding protein	61 kDa	Sugar ABC transporter	Secreted
C5VW19	SSU1869	Extracellular metal cation-binding protein	36 kDa	ZnuA / Zinc uptake	Secreted
C5VZZ1	SSU0465	Putative exported protein	41 kDa	Predicted membrane fusion protein	Membrane
C5VYV7	SSU0284	Extracellular solute-binding protein	35 kDa	ABC transporter	Periplasm
C5VYN9	SSU1364	Branched-chain amino acid ABC transporter	41 kDa	ABC transporter	Periplasm
Protein biosynthesis (n = 2)
C5VXU6	SSU1196	Threonine-tRNA ligase	75 kDa	ThrS / protein biosythesis	Cytoplasm
C5VZL9	SSU0412	Valine-tRNA ligase	101 kDa	ValS / protein biosynthesis	Cytoplasm
Carbohydrate/sugar metabolism (n = 10)
C5VXF6	SSU1127	Putative N-acetylmuramoyl-L-alanine amidase	113 kDa	Peptidoglycan catabolism	Unknown
C5VY36	SSU0153	Glyceraldehyde-3-phosphate dehydrogenase	39 kDa	Plr / adhesin	Secreted
C5VWC9	SSU1915	Putative maltose/maltodextrin-binding protein	44 kDa	sugar binding	Membrane
C5VWE7	SSU1933	Putative fumarate reductase flavoprotein subunit	53 kDa	Fumarate reductase flavoprotein subunit	Unknown
C5VXV7	SSU1207	Putative lipoprotein	30 kDa	Predicted glucose-6-phosphate isomerase	Unknown
C5VVZ9	SSU1849	Putative surface-anchored amylopullulanase	234 kDa	ApuA / amylopullulanase / Adhesin	Cell wall
C5VY37	SSU0154	Phosphoglycerate kinase	42 kDa	Phosphoglycerate kinase	Cytoplasm
C5VYJ5	SSU1320	Enolase	47 kDa	Eno / adhesin	Cell wall
C5VYP7	SSU1372	Multiple sugar-binding protein	45 kDa	Sugar transporter	Unknown
C5VZ34	SSU1451	2,3-bisphosphoglycerate-dependent phosphoglycerate mutase	26 kDa	GpmA / glycolytic process	Unknown
Nucleotide metabolism (n = 1)
C5VX44	SSU1044	Ribonucleoside-diphosphate reductase	82 kDa	DNA synthesis	Cytoplasm
Protein folding/arranging (n = 2)
C5VX75	SSU1078	Foldase protein PrsA	38 kDa	PrsA / protein folding	Membrane
C5VXK4	SSU1177	Peptidyl-prolyl cis-trans isomerase	28 kDa	Protein folding	Unknown
Gene expression (n = 4)
C5W008	SSU0482	Elongation factor Tu	44 kDa	TufA / elongation factor Tu	Cytoplasm
C5VY08	SSU0123	DNA-directed RNA polymerase subunit beta	136 kDa	DNA-dependant RNA polymerase	Cytoplasm
C5VY34	SSU0151	Elongation factor G	77 kDa	FusA / elongation factor G	Cytoplasm
C5VY07	SSU0122	DNA-directed RNA polymerase subunit beta	133 kDa	RpoB / RNA-polymerase	Cytoplasm
Catalytic activity (n = 5)
C5VVK9	SSU1773	Putative surface-anchored serine protease	187 kDa	SspA / Subtilisin-like protease	Cell wall
C5VVJ6	SSU1760	Surface-anchored DNA nuclease	114 kDa	SsnA / Nuclease	Cell wall
C5W022	SSU0496	Putative Mac family protein	124 kDa	IgM protease	Membrane
C5W049	SSU1616	Putative exported protein	122 kDa	Predicted ribonuclease G and E	Unknown
C5VXH2	SSU1143	Putative surface-anchored zinc carboxypeptidase	118 kDa	Surface anchored zinc carboxypeptidase	Cell wall
Defense/virulence mechanisms (n = 4)
C5VYR5	SSU1390	Streptococcal histidine triad-family protein	96 kDa	HtpsC	Cell wall
C5VVS8	SSU0706	Muramidase-released protein	136 kDa	Mrp	Cell wall
C5VZA8	SSU0370	Putative penicillin-binding protein 1A	79 kDa	Pbp1A / Penicillin-binding protein	Unknown
C5VXY1	SSU1231	Suilysin	55 kDa	Sly / Hemolysin	Secreted
Ion binding (n = 1)
C5VY00	SSU0115	Zinc-binding protein AdcA	56 kDa	AdcA/Zinc-binding protein	Unknown
Redox (n = 1)
C5VYT4	SSU0261	Aldehyde-alcohol dehydrogenase	97 kDa	Oxydoreductase	Unknown
Uncharacterized proteins (n = 4)
C5VV38	SSU0595	Putative exported protein	13 kDa		Unknown
C5VZS6	SSU1560	Putative lipoprotein	17 kDa		Unknown
C5VV37	SSU0594	Putative exported protein	14 kDa		Unknown
C5VV35	SSU0592	Putative exported protein	12 kDa		Unknown