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. 2015 Jun 25;11(6):e1005047. doi: 10.1371/journal.pgen.1005047

Fig 5. General effect of Kre on gene expression.

Fig 5

(A) Absence of kre leads to increased GFP expression from a Pxyl promoter. Strains PG537 (amyE::Pxyl-gfp, ΔcomK, ΔmecA) and PG538 (amyE::Pxyl-gfp, ΔcomK, ΔmecA kre:Tn) were grown to logarithmic phase in LB at 37°C in the presence of 0.1% xylose. Graph shows the results of one representative experiment (3 biological replicates). (B) Absence of kre leads to increased GFP expression from the Physp promoter in a wild type background. Strains PG820 (amyE::Physp-gfp) and PG821 (Physp-gfp, kre:Tn) were grown to logarithmic phase in LB at 37°C in the presence of 50 μM IPTG. Graph shows the results of one representative experiment (3 biological replicates). (C) Increase in β-galactosidase expression when kre is deleted. PG500 (amyE::Pveg-lacZ-gfp), PG512 (amyE::Pveg-lacZ-gfp, Δkre), PG811 (amyE::PpksA-lacZ-gfp), PG815 (amyE::PpksA-lacZ-gfp, Δkre) were grown in LB at 37°C and samples were collected at O.D.600 ~0.2–0.3 for β-galactosidase activity measurements. Graphs show the ratio between kre mutant and wild-type strain averaged over 3 independent experiments.