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. 2015 Jun 25;11(6):e1005047. doi: 10.1371/journal.pgen.1005047

Fig 6. Kre affects comK mRNA stability.

Fig 6

(A) Relative increase in veg, pksA and comK mRNA levels in a kre mutant determined by quantitative real-time PCR (qPCR). RNA was isolated from PG500 (amyE::Pveg-lacZ-gfp) and PG512 (amyE::Pveg-lacZ-gfp, Δkre), and results shown are the average of 3 biological replicates. (B & C) Strains PG500 (amyE::Pveg-lacZ-gfp) and PG512 (amyE::Pveg-lacZ-gfp, Δkre) were grown in LB at 37°C. At OD600 ~0.2 T0-samples were collected immediately before rifampicin (150 μg/ml) was added. Subsequent samples were taken 2, 4, 6, 8 and 16 min after rifampicin addition. Relative abundance of comK (B) and ftsZ (C) transcripts were quantified over 3 independent experiments using qPCR. (D & E) Strain PG474 (amyE::Physp-kre) was grown in LB at 37°C in the presence or absence of 1 mM IPTG. At OD600 ~0.25, T0-samples were collected immediately before rifampicin was added. Subsequent samples were taken 1, 2, 3, 4, 6, and 8 minutes after rifampicin addition. Relative abundance of comK (D) and ftsZ (E) transcripts were quantified over 3 independent experiments using qPCR.