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. 2015 Jun 25;11(6):e1005047. doi: 10.1371/journal.pgen.1005047

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© 2015 Gamba et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 8 — (A) Schematic representation of the kre promoter region. Three potential AT-boxes and the putative -35 promoter region [38] are highlighted in red and grey, respectively. (B) Overproduction of ComK in a mecA mutant causes repression of kre expression. Strains PG501 (amyE::Pkre-lacZ-gfp), PG763 (amyE::Pkre-lacZ-gfp, ΔmecA), PG764 (amyE::Pkre-lacZ-gfp, ΔcomK) and PG765 (amyE::Pkre-lacZ-gfp, ΔmecA, ΔcomK) were grown in competence medium at 37°C, and samples were collected at OD₆₀₀ ~0.1 for β-galactosidase measurements. (C) Schematic representation of the double negative feedback regulation exerted by Kre and ComK. (D) Reciprocal correlation between Pkre and PcomG expression. Strain PG688 (kre:Pkre-gfp, amyE::PcomG-mcherry) was grown in competence medium at 37°C, and phase contracts and fluorescent images were taken after overnight incubation. (E) Average GFP and mCherry levels in single cells from the same culture in D (n = 211).