Table 1. Dissociation constant (Kd) and stoichiometry (n) of S15 to wild type ED3 and mutants.
rED3 | S15 | |
---|---|---|
Dissociation constant | Number of binding site (n) | |
WT | 200 | 1.182 |
I335A | 279 | 1.154 |
R345A | 190 | 1.181 |
Q316G | 860 | 1.099 |
H317A | 1027 | 1.074 |
G318A | 606 | 1.095 |
G318P | 1008 | 1.079 |
T319A | 378 | 1.139 |
I320A | 867 | 1.094 |