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. 2015 Jun 25;10(6):e0130360. doi: 10.1371/journal.pone.0130360

Fig 1. Detection limit using FCBA BCR-ABL1 protein serial dilution curve.

Fig 1

The CML cell line K562 was diluted in the BCR-ABL-negative cell line, HL60. Negative cut-off median PE value for this series was 105. Assay sensitivity was compared with real-time quantitative PCR (RQ-PCR) of BCR-ABL1 transcripts for each dilution and standardized according to the International scale (IS). The protein assay sensitivity indicated a loss of sensitivity of the FCBA assay at a dilution between 0.1 and 0.01% K562 which corresponds to a %BCR-ABL1/ABLIS mRNA ratio between 0.054 and 0.60%.