Table 1. Comparison of Flow-Cytometric Bead Assay (FCBA) vs. RQ-PCR for Detection of BCR-ABL1 Expression in CML Follow-Up.

Molecular response	FCBA-Negative	FCBA-Positive
(According to ELN guidelines)	n samples (RQ-PCR range)	n samples (RQ-PCR range)
>10%	0	39 (10.258–195.807)
≤10–1% CHR	10 (1.044–3.769)	31 (1.198–9.914)
≤1–0.1% CCyR	26 (0.121–0.771)	5 (0.143–0.519)
≤ 0.1% MMR	37 (0–0.100)	2 (0.028–0.073)

N = 150 serial samples, belonging to 54 CML in follow-up* (*note that 1 CML patient with p230 transcripts was not included in this analysis).

Abbreviations: FCBA, Flow-cytometric Bead Assay, CHR = complete hematologic remission, CCyR = complete cytogenetic remission, MMR = major molecular response; (RQ-PCR) = quantitative real-time PCR; ELN = European Leukemia Net [8]