Fig 5. Combined treatment effect of PROG and TMZ on U87MG cell migration in a wound healing assay.

Cells were grown in multi-well plates and pre-treated with (A) PROG alone and (B) in combination with TMZ at different concentrations for 2 h. A scratch/wound was formed with a 200-μl tip and the cells were incubated with PROG, TMZ or their combinations for the next 24 h. Photographs (4x) were taken at 0 h and 24 h post-wound formation. In the vehicle group, a large number of cells migrated from both sides to heal the wound at 24 h compared to 0 hr. PROG and TMZ individually decreased U87MG cell migration 24 h after wound formation compared to vehicle. The combination of the highest concentrations of both the drugs inhibited cell migration better than either drug alone. Representative photomicrographs from three separate replication experiments (n = 3 each).