Fig 7. Effect of PROG and TMZ on the proliferation and the expression of MGMT in U87MG and U118MG cells.

Tumor cells (U87MG and U118MG) were seeded (0.5 x 10⁶) in 60-mm petri dishes and kept under starvation overnight prior to drug exposure. Cells were repeatedly exposed to different concentrations of PROG and/or TMZ for 3 days. Protein samples (50 μg) were separated under reducing and denaturing conditions by 4–20% acrylamide Criterion gel and analyzed for EGFR, pAkt, total Akt and mTOR expression. The density of each P-Akt band was corrected for variance in loading, using the density of the corresponding total Akt. β-actin was used as a loading control for densitometry. (A) Representative Western blot and densitometric analysis of the expression of proliferation marker PCNA. (B) Expression of MGMT in U87MG and U118MG cells, and (C) Inhibitory effect of PROG on MGMT in U118MG cells. Data are expressed as means ± SD from two separate replication experiments (n = 3 samples each). Statistically significant difference: *P<0.05 compared to control; ^# P<0.05 compared to T100 alone.