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. 2015 Jun 25;10(6):e0131271. doi: 10.1371/journal.pone.0131271

Fig 4. Detection of siRNAs based on the bidirectional RNA expression driven by the left and right DIRS-1 ITRs.

Fig 4

(A) Northern blot to detect alpha-Actinin (abpA), Coronin (corA) and Severin (sevA) siRNAs. Probes at the trigger-ends and near the trigger-centres are indicated and correspond to the probes shown in Fig 3 (see also S1 Table). (B) Northern blot to detect casein kinase (casK), cullin D (culD), queuine tRNA-ribosyl transferase 1 (qtrt1) siRNAs at the end of the trigger. A mixture of radiolabelled oligonucleotides (position indicated in Fig 3, see also S1 Table) was used for hybridization. All samples in A and B respectively were run on the same gel. Probes against snoRNA DdR-6 and DIRS–1 siRNAs were separately used as loading controls for A and B.