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. 2015 Jun 25;10(6):e0131271. doi: 10.1371/journal.pone.0131271

Fig 7. Comparison of published knock-out phenotypes with knock-downs by ITR constructs.

Fig 7

(A) Nuclei count in Ax2 wild type cells and different knock-down strains. Knock-down of corA (lTR corA rITR) resulted in increased multinuclearity. 200 cells of each strain of two independent transformations were counted. (B) Knock-down of mhcA by an ITR construct showed huge cells containing up to 30 nuclei and more compared to Ax2 wild type and to a control knock-down of abpA. Scale bar 50μm. (C) Growth curve of Ax2 wild type cells and different knock-down strains. Knock-down of casK resulted in a slow growing culture. Measurement of cell density in the indicated cell lines over 72 hours. Data are plotted as relative cell count over time. Ax2, lITR GFP rITR, lITR abpA rITR: n = 2; lITR casK rITR: n = 4.