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. 2015 Jun 26;10(6):e0130478. doi: 10.1371/journal.pone.0130478

Fig 2. Chemical cross-linking HMGA2 into homodimers with EDC.

Fig 2

Chemical cross-linking reactions with EDC in MES buffer were performed as described under “Materials and Methods.” Cross-linked protein samples were analyzed by electrophoresis in a 15% SDS-PAGE gel and stained with Coomassie Brilliant Blue R-250. Lane 1 contained molecular standards; lane 2 contained HMGA2 in the absence of EDC; lanes 3 to 6 contained, respectively, 29, 39, 58, and 116 μM HMGA2 in the presence of 2 mM EDC. M, monomer; D, dimer; T3, trimer; T4, tetramer.