Fig 5. Chemical cross-linking of HMGA2 and HMGA2Δ95–108 with EDC.

Chemical cross-linking reactions with EDC in MES buffer were performed as described under “Materials and Methods.” Cross-linked protein samples were analyzed by electrophoresis in a 15% SDS-PAGE gel and stained with Coomassie Brilliant Blue R-250. Lane 1 contained HMGA2Δ95–108 in the absence of EDC; lanes 2 and 3 contained 40 μM HMGA2Δ95–108 in the presence of 2 mM EDC; lane 4 contained HMGA2 in the absence of EDC; lanes 5 and 6 contained 40 μM HMGA2 in the presence of 2 mM EDC; lane 7 contained molecular standards.