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. 2015 Jun 26;10(6):e0129016. doi: 10.1371/journal.pone.0129016

Table 4. Selected de novo motifs found in SKn dehydrin promoters and their putative function identified through PLACE database.

Match in PLACE 5
De novo motif Sequence Name E-value 6 Function
5. Seeder 1 MCACGTGTC 2 (120/120 3 , 9.2e-19 4 ) GGACACGTGGC ABRETAEM 6.5e-13 ABRE found in wheat
TGACACGTGGCA HY5AT 2.4e-12 Bound by HY5, involved in light regulation of transcriptional activity
TCCACGTGTC SGBFGMGMAUX28 1.1e-13 Recognized by G-box binding factors in soybean. Found in auxin-responsive genes
MCACGTGGC GBOXLERBCS 8.1e-12 Sequence found in promoters of light-regulated genes
CGCCACGTGTCC ABREBNNAPA 2.4e-21 ABRE found in Brassica napus
6. MEME CCGACGCG (120/120, 1.2e-50) CCCACGTGGC ABREAZMRAB28 3.0e-05 ABRE, ABA and water-stress responses. Binding site of CBF2.
GCCGCGTGGC ABREMOTIFIIIOSRAB16B 4.0e-05 ABRE Motif III found in rice
CCACGTGGCC LTRECOREATCOR15 5.3e-06 LTRE
7. Seeder SCAACGCG (120/120, 2.0e-10) TCCACGTCTC ABRE3HVA1 2.3e-05 ABRE found in barley
GCCGCGTGGC ABREMOTIFIIIOSRAB16B 6.0e-05 ABRE Motif III found in rice
8. MEME CACCGACC (119/120, 3.8e-59) RCCGAC DRECRTCOREAT 1.9e-08 DRE/CRT found in genes expressed in response to cold and dehydration
CCCACCTACC ACIPVPAL2 3.8e-07 Required for vascular specific expression
ACCGACA LTREATLTI78 3.1e-07 LTRE
9. Seeder KKGTCGGY (120/120, 4.9e-07) ACCGACA LTREATLTI78 8.4e-07 LTRE
RCCGAC DRECRTCOREAT 2.4e-08 DRE/CRT found in genes expressed in response to cold and dehydration
10. MEME GTGGGVCC (61/120, 2.5e-22) GGTCCCAT GGTCCCATGMSAUR 8.3e-09 Auxin RE found in soybean
CTCCCAC BOXCPSAS1 3.9e-06 Involved in light-induced repression

1Number of the motif and the de novo discovery software that was used to locate that motif.

2Motif consensus sequence in IUPAC nucleotide code.

3Occurrence is the number of promoters containing a de novo motif out of the total number of promoters analyzed for a specific dehydrin class, presented in the parentheses.

4Siginificance of the motif, E-value calculated by MEME, Q-value calculated by Seeder, presented in the parentheses.

5PLACE matches were identified using STAMP, only significant matches with E-value < 0.05 are presented.

6E-value of the match with PLACE motif.