Table 1. Bacterial strains and plasmids.

Strains	Relevant Features	Reference
B. mallei ATCC 23344	Human clinical isolate; KmSPbR	[54]
B. pseudomallei K96243	Human clinical isolate; KmRGmRZeoR PbR	[55]
B. mallei CSM001	B. mallei ATCC 23344 with a mini-Tn5::luxKm2; KmRPbR	[25]
B. mallei TMM001	B. mallei ATCC 23344 with an unmarked deletion of bmaa1801 (∆tonB)	This study
B. mallei TMM002	∆tonB complemented with pTonB-comp; KmR	This study
E. coli S17-1	For conjugal transfer, recA thi pro hsdRM+ RP4:2-Tc:Mu:KmTn7 TpR SmR	[56]
E. coli S17-1(pTonB-allex)	E. coli S17.1 with the recombinant suicide plasmid pTonB-allex; KmR	This study
Plasmids
pMo130	Suicide vector for allelic exchange in Burkholderia; used to construct pTonB-allex; pUC19 ori, RK2 oriT, xylE, sacB, KmR	[22]
pTonB-allex	pMo130 derived recombinant suicide plasmid used to generate ∆tonB; KmR	This study
pMo168	Replicative vector for Burkholderia; oripBBR1, mob+, xylE, KmR	[22]
pTonB-comp	pMo168 derived recombinant replicative vector used to complement ∆tonB; pMo168:: bmaa1801; KmR	This study