Table 1. DNA oligonucleotides used for cloning WNV NS5 into bacterial expression plasmid.
DNA Oligonucleotide | Sequence | |
---|---|---|
1 | Ub-SacII-INF-WNV-NS5-for | 5’TG GTC CTG CGT CTC CGC GGT GGA GGT GGG GCA AAA GGA CGC3’ |
2 | WNV-NS5-INF-BamHI-CHIS-rev | 5’G GTG ACC AGA GGA TCC CAG TAC TGT GTC CTC AAC-3’ |