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. 2015 Jun 26;10(6):e0128472. doi: 10.1371/journal.pone.0128472

Fig 4. MiR-124 overexpression reduces DNA SB repair capacity.

Fig 4

(A) HR repair capacity in miR-124-overexpressing cells and cells transfected with the antisense microRNA control (anti-miR-CON) or antisense miR-124 inhibitor (anti-miR-124). (B) Comet assay showing that miR-124 overexpression (miR-124) reduces SB repair relative to the vector control (VC) after treatment with CPT. (C) Immunostaining of DNA SBs (detected by the presence of γ-H2AX) in cells after IR treatment. Red: anti-γ-H2AX; blue: nuclei (left panel). The γ-H2AX signal was quantified and compared with the cells transfected with VC or miR-124 (right panel). All of the experiments shown were conducted in U-2 OS cells, and similar results were obtained in MDA-MB-231 cells. *P < 0.05 in individual comparisons.