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. Author manuscript; available in PMC: 2015 Jun 26.
Published in final edited form as: Eur J Immunol. 2014 Feb 19;44(4):1119–1129. doi: 10.1002/eji.201343866

Figure 1.

Figure 1

A2AR stimulation inhibits induction of acute hepatitis and activation of NKT cells. (A–C) Effect of A2AR agonist CGS21680 (CGS) on Con A-induced liver injury. C57BL/6 mice received i.p. injection of CGS (0.5 mg/kg) 10 min prior to Con A administration (12 mg/kg i.v.). Serum ALT levels (A) were determined after 8 h. Serum IL-4 (B) and TNF-α (C) levels were determined after 1.5 h. (D) Gating strategy for flow cytometric analysis of NKT cells in liver mononuclear cells. (E, F) Representative density plots of CD69 (E) and CD40L (F) expression on NK1.1+ CD3+ cells in mice liver 1.5 h after Con A injection. “None” means untreated control. Numbers represent percentages of gated cells. ** P < 0.01, *** P < 0.001 vs Con A alone; Student’s t-test. Data represent average ± SD of 5 mice (A–C) or 3 mice (E, F) and are representative of 3 (A–C) and 2 (E, F) independent experiments.