FIG. 8.

Acetaminophen response of HepG2 cells under different culturing conditions. (a) Representative images of HepG2 cells cultured 10 days in the perfused chip and exposed to different concentrations of acetaminophen. (b) Dose-response curve of HepG2 cells exposed to acetaminophen under different culturing conditions. Data are shown as mean ± SD (n = 3). Significant differences (p < 0.05) are indicated as follows: * 2D vs. chip culture, ^$static 3D vs. chip culture, ^& 2D vs. static 3D culture at the respective concentration, and ^# within 2D, static 3D, and chip cultures vs. their respective baseline (0.5 mM).