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Amplification strategy of HIV-1 NFLG. (a) HIV-1 gene map in HXB2 [GenBank:K03455] co-ordinates Adapted from Los Alamos Database (www.hiv.lanl.gov). (b) The locations of all primers used for cDNA synthesis, nested and semi-nested PCR are shown. The 8.8 kb genome is amplified into two fragments: fragment 1, 5.5 kb (gag-vpu) and fragment 2, 3.7 kb (vif-3′-LTR) with 400 bp overlap. (c) Representative amplification of all four subtypes is presented.
