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. 2014 Sep 21;17(4):526–535. doi: 10.1093/neuonc/nou229

Fig. 3.

Fig. 3.

Immunoprecipitation (IP), reverse IP, followed by Western blotting analyses were performed on the ATRT cell lines, CHLA-04-ATRT (04), and CHLA-05-ATRT (05). The appropriate proteins that were detected for these analyses are indicated beside the blots along with their molecular weights. For each cell line, a cell lysate (CL) sample and immunoprecipitated (IP) sample were used as indicated above each lane in the blots. (A) As controls, Wnt5B IP samples from each cell line were analyzed, along with a β-catenin IP and a Wnt5B IP with media. (B) IPs with 2 cell lines using Wnt5B antibody. The CL and IP samples were blotted using antibodies against Frizzled 1, 2, 3, and 7. A reverse IP with cell lines using Frizzled 1, 2, 3, and 7 antibodies each bound to an IP column. These IP samples were blotted to detect Wnt5B protein as indicated. (C) IPs with 2 cell lines using Wnt5B antibody. The CL and IP samples were blotted with a Ryk antibody. (D) IPs with 2 cell lines using Wnt5B antibody. The CL and IP samples were blotted with a Ror1 antibody.