Figure 3. SLN deficiency promotes reduction in cellular respiratory rates.
A: Representative images of sc and shSLN C2C12 cells. SLN knockdown did not affect differentiation efficiency or morphology. B: Quantitative PCR of SLN in sc and shSLN C2C12 myotubes. C: Cellular respiration rates of sc and shSLN C2C12 myotubes. D: Mean values for four phases of respirations. All data were collected from day 4 of differentiation. Filled bars/dots: sc, empty bars/dots: shSLN. sc: scrambled, shSLN: short-hairpin SLN (SLN-knockdown). n=6/group. *: p < 0.05. Data are shown mean ±SEM.