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. 2015 Jun 24;8:92. doi: 10.1186/s13068-015-0270-7

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© Tao et al. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Engineered pathways for the production of FABCEs. Branched-chain alcohols (isobutanol and isoamylol) were produced through the branched-chain amino acid biosynthetic pathway by overexpression of alsS, ilvC, ilvD, aro10, and adh2. FABCEs were synthesized through esterification of branched-chain alcohols and fatty acyl-CoAs by overexpression of ws/dgat. Furthermore, BFABCEs were produced by overexpression of ws/dgat which can catalyze esterification of branched-chain alcohols and branched fatty acyl-CoAs produced by the overexpression of bckd and fabHB