Table 3.
Primers used for PCR amplification of genes
| Primer name | Primer sequence (5′-3′) |
|---|---|
| ilvC-XbaI | GTATCTAGAAAGAGGAGATATAATGGCTAACTACTTCAATACACTGAATCTG |
| ilvC-SpeI-BamHI | ACTGGATCCACTAGTTTAACCCGCAACAGCAATACGTT |
| ilvD-XbaI | GTATCTAGAAAGAGGAGATATAATGCCTAAGTACCGTTCCGCCA |
| ilvD-SpeI-BamHI | ACTGGATCCACTAGTTTAACCCCCCAGTTTCGATTTATCG |
| BCKD-SacI | AGATGAGCTCATGGCAACTGAGTATGACGTAGTCATTC |
| BCKD-SpeI | AGTACTAGTTTAGTAAACAGATGTCTTCTCGTCAATCG |
| fabHB-BamHI | ACTGGATCCAAGAAGGAGATATAATGTCAAAAGCAAAAATTACAGCTATCGG |
| fabHB-XhoI | TGTCTCGAGTTACATCCCCCATTTAATAAGCAATCCTG |
| WS/DGAT-SfuI | AGGTTTCGAAATGAGACCATTACACCCTATTGATTTC |
| WS/DGAT-EcoRI | GACGAATTCTTAGTTGGCAGTCTTAATGTCTTCTTG |
| HIS4-BamHI | AATGGATCCCTCGCAGCTGATGAATATCTTGTG |
| HIS4-BglII | CAAGGATCCACCGATACCAGGATCTTGCCAT |
| ARO10-SfuI | GCGTTCGAAATGGCACCTGTTACAATTGAAAAGTTC |
| ARO10-EcoRI | CCGTCTAGACTATTTTTTATTTCTTTTAAGTGCCGCTG |
| ADH2-SfuI | CCGTTCGAAATGTCTATTCCAGAAACTCAAAAAGCC |
| ADH2-EcoRI | GCGGAATTCTTATTTAGAAGTGTCAACAACGTATCTACCAG |