Fig 5. Incorporation of branched stem peptides in PG inversely correlates with Ply release.

(A) Ratio of branched to linear stem peptides in the PG of indicated strains. (B) Percentage of oligomers (dimers and trimers) containing a crossbridge as defined by a crosslink that directly incorporates the branch moiety. (C-E) Percent Ply release was calculated using the data from Figs 2B, 2C, 4A and 4B and plotted against the percentage of total branched stem peptides (C), branched monomers (D), and branched oligomers (E). Each symbol represents a given strain. Statistical dependence between percent Ply release and each variable was determined by calculating Pearson’s correlation coefficient (r). In all panels, data are presented as the mean ± SEM.