Skip to main content
. 2015 Jun 26;10(6):e0131281. doi: 10.1371/journal.pone.0131281

Fig 1. Overexpression of α-syn in the SNpc after rAAV-α-syn transduction.

Fig 1

Western blotting showed a significant expression of human-α-syn in HEK293 cells 96 hours after transduction (A). The position of SNpc was indicated (B) and TH positive staining (Red) indicated a loss of DA neurons in the SNpc 8 weeks after rAAV-α-syn injection, however, the co-staining of human-αsyn (Green) was also observed in the existing DA neurons (I-K). The overexpression of GFP was also detected in TH-positive neurons in the SNpc after rAAV2/1-GFP injection (F-H). And no α-syn staining was observed in the none-injected mouse (C-E). TH staining confirmed no loss of DA neurons in GFP and none injected group. Antibody against human α-syn was used (CST). Scale bar: 100μm.