Fig. 1.
sCLU depletion in U-2 OS cells induces growth arrest and increased cell death responses. A1 quantitative PCR analyses (n = 3) of sCLU mRNA levels after cell transfection with indicated siRNA oligonucleotides. A2, immunoblot analyses of sCLU protein levels in whole-cell lysates following transfection with shown siRNAs. Actin probing was used as reference for equal protein loading; molecular weight markers are shown to the right of the blot. B, DNA synthesis measurement (n = 2) after bromodeoxyuridine incorporation. C, cumulative cell death quantitation (n = 3) of DNA fragments following cell transfection with the indicated siRNAs. D, comparative fluorescence-activated cell sorting analysis (representative of two independent experiments) of control (Sc-I), Cl-I, or sCLU siRNA-treated cells. Assays were done 72 h after siRNA cell transfection. Bars, SD. *, P < 0.05.