Table 2.
Evaluated parameters of cell quality on day 2 (The data represented mean±SD). Effects of UV alone in different doses on platelet quality (1A). The effects of RB (50 μM) alone and RB in combination with UV light on the platelet quality (1B)
| A | ||||||
|---|---|---|---|---|---|---|
| C-PC | CUV-PC | |||||
| J/cm2 | 0.24 | 0.48 | 0.97 | 1.29 | ||
| 2 min | 4 min | 8 min | 10 min | |||
| Cell count (×103/μl) | 740±7.07 | 707.5±17.67 | 695±63 | 597.5±81.31 | 565±77.79 | |
| pH | 7.9±0.15 | 8.07±0.12 | 8.04±0.04 | 8.01±0.16 | 8.02±0.11 | |
| LDH (U/L) | 1362.55±188.8 | 1549.54±75.6 | 1870.22±453.3 | 1469.47±340 | 1319.51±325.4 | |
| MTT assay (OD 575 nm) | 2.92±0.5 | 2.94±0.5 | 2.4±0.01 | 2.7±0.8 | 2.4±0.6 | |
| B | ||||||
|---|---|---|---|---|---|---|
| C-PC | CRB50 μM-PC | Treatment with RB (50 μM)+UV | ||||
| J/cm2 | 0.24 | 0.48 | 0.97 | 1.29 | ||
| 2 min | 4 min | 8 min | 10 min | |||
| Cell count (×103/μl) | 727±180.3 | 632±208.6 | 585±268.7 | 600±290 | 485±247.4 | 410±162.6 |
| pH | 7.96±0.007 | 7.91±0.14 | 8±0.21 | 7.95±0.23 | 8.02±0.12 | 7.96±0.12 |
| LDH (U/L) | 1496.08±453.3 | 1148.87±340 | 1656.44±226.7 | 1335.86±302.3 | 1309.09±188.9 | 1309.09±264.4 |
| MTT assay (OD 575) | 3.4±0.27 | 2.9±0.6 | 1.9±0.28 | 2.8±0.4 | 2.6±0.7 | 2.5±1.7 |