Table 2.

Validated Fusion Transcripts Present in Healthy Organ Donors

Fusion gene	Protein product	Head/tail gene locus	Distance	Head/tail joining sequence	Age/number
HARS2-ZMAT2	Head gene-WT	5q31.3/5q31.3	2 kb	5′-CGTTGTCAAGAG-3′	19–71/9
	Tail gene-AWTASC			5′-ACAAAAAACTTG-3′
BAIAP2L2-SLC16A8	Head gene-WT	22q13.1/22q13.1	1.9 kb	5′-GGGGTCCGAGAC-3′	19–71/9
	Tail gene-WT			5′-CTGCTCCACTTC-3′
KLK4-KLKP1	Head gene-CTT	19q13.1/19q13.1	20 kb	5′-TGCTGGCGAACG-3′	19–71/10
	Tail gene-NC			5′-ATGCTGTGATTG-3′
AZGP1-GJC3	Chimera protein	7q22.1/7q22.1	48 kb	5′-ACGACAGTAACG-3′	19–71/10
	Chimera protein			5′-TTCCAGGAAGAA-3′
SLC45A3-ELK4	Chimera protein	1q32.1/1q32.1	38 kb	5′-CGGGAGAAGCAG-3′	19–71/9
	Chimera protein			5′-CTCATTGCTATGG-3′
PRKAA1-TTC33	Head gene-CTT	5p13.1/5p13.1	17 kb	5′-GTAGTATTGATG-3′	19–71/10
	Tail gene-WT			5′-AATGGCTTCCTT-3′
C6orf47-BAG6	Head gene-AWTASC	6p21.3/6p21.3	8.5 kb	5′-GGTGTGGCCTTG-3′	19–71/10
	Tail gene-WT			5′-AGACCTGTCGGC-3′
ADCK4-NUMBL	Chimera protein	19q13.2/19q13.2	6 kb	5′-TTTGAAACCAAG-3′	22–71/7
	Chimera protein			5′-GGCGGACCCCGG-3′
TTTY15-USP9Ya	Head gene-NC	Yq11.1/Yq11.1	15 kb	5′-TTCTGACCTGTG-3′	15–75/19
	Tail gene-WT			5′-AGAGCTTGGAG-3′
TTTY15-USP9Yb	Head gene-NC	Yq11.1/Yq11.1	15 kb	5′-GTATTTTGGAAG-3′	15–56/16
	Tail gene-WT			5′-AGAGCTTGGAG-3′
MALAT1-TNN	Head gene-NC	11q13.1/2q31	Infinite	5′-ATGGGACAATAG-3′	21/1
	Tail gene-AWTASC			5′-TAATGTCGCTGG-3′
MALAT1-WDR74a	Head gene-NC	11q13.1/11q12.2	2.6 mb	5′-CAGTAAGAAAAA-3′	21/1
	Tail gene-WT			5′-ATCAAGTGTAGT-3′
MALAT1-WDR74b	Head gene-NC	11q13.1/11q12.2	2.6 mb	5′-TGGGTTAGAGAA-3′	21/1
	Tail gene-AWTASC			5′-GGAGTCTCACTC-3′

Transcripts were validated through RT-PCR and Sanger sequencing.

AWTASC, absence of wild-type ATG start codon; CTT, C-terminal truncation; NC, non-coding; WT, wild-type.