Figure 4.

Expression of Fgfr3 and Fgfr4 increases in a high-glucose medium in vitro and in a human specimen. A: Fgfr3 and Fgfr4 mRNA expression levels in a cultured mouse podocyte cell line and mesangial cell line after three different administrations of a 72-hour glucose challenge [90 (white bars), 270 (gray bars), or 450 mg/dL (black bars)] are shown. Osmolality was controlled using d-mannitol. B: Immunofluorescence staining of the human kidney is shown. Lanes from the left: Nondiabetic control, patient with mild diabetes mellitus nephropathy (DN), and patient with severe DN. Top panels are representative images in periodic acid-Schiff (PAS) stain. FGFR3 and FGFR4 (green) were stained with Alexa 488 fluorescence. Arrows depict increased expression of FGFR in the glomerulus. Data are expressed as means ± SEM (A). n = 6 (A). Post hoc ^∗P < 0.05, ^∗∗P < 0.01 versus 90 mg/dL.