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. 2015 Jul;185(7):1889–1898. doi: 10.1016/j.ajpath.2015.03.025

Table 2.

Quantitative Real-Time PCR Primer Sequences

Target gene Primer sequence
Mouse Gpc5 F: 5′-CTAGTGGAGACTGTGATGACGAA-3′
R: 5′-TTCATGCTGTCTGGCATCC-3′
Probe#76 (Roche)
Mouse Fgfr3 F: 5′-GGAGGACGTGGCTGAAGA-3′
R: 5′-CGGAAGCGGACAGTGTTT-3′
Probe#76 (Roche)
Mouse Fgfr4 F: 5′-ATGACCGTCGTACACAATCTTAC-3′
R: 5′-TGTCCAGTAGGGTGCTTGC-3′
Mouse Fgf2 F: 5′-CGGCTCTACTGCAAGAACG-3′
R: 5′-TGCTTGGAGTTGTAGTTTGACG-3′
Rat Fn1 F: 5′-CCGGTGGCTGTCAGTCAGA-3′
R: 5′-CCGTTCCCACTGCTGATTTATC-3′
Rat Col4a2 F: 5′-TGATGGAGAAAAAGGAGTCTCAG-3′
R: 5′-GCCTTGGAAACCATCCAG-3′
Mouse Actb F: 5′-CGCACCACTGGCATTGTCAT-3′
R: 5′-TTCTCCTTGATGTCACGCAC-3′
Rat Gapdh F: 5′-CAAGGTCATCCATGACAACTTTG-3′
R: 5′-GGCCATCCACAGTCTTCTGG-3′

F, forward primer; R, reverse primer.