Figure 5.

FGF-23 signaling pathway is downregulated in proteinuric rats. Protein expression of FRS2α and its phosphorylation level (p-FRS2α) as well as PKA substrates phosphorylation (p-PKA substrate) levels were assessed by Western blot of cortical lysates in control (Ctl) and PAN rats submitted to a normal- (A) or high-phosphate (HP) diet (B). Representative Western blot of FRS2α, p-FRS2α, and p-PKA substrate (upper panel: 3 animals per group). Bars (lower panel) show densitometric quantification of Western blots (n=6 per group in A) and (n=9 per group in B). Results are expressed as the mean ratio of individual values over the mean value obtained in Ctl±SEM. *P<0.05.