Figure 6.
Transgenic mice harboring glomerular proteinuria display increased NaPi-IIa and decreased Klotho expression. Biochemical parameters measured in control wild-type (open bars) and POD-ATTAC proteinuric mice (closed bars) (A) (n=5 per group). *P<0.05. P04, plasma phosphate concentration. All parameters were measured in the same set of mice except for PTH, which was measured on a separate set for technical reasons (n=5 and 10). NaPi-IIa, Klotho, FRS2α, and its phosphorylated form (p-FRS2α) were detected by Western blot of kidney lysates in wild-type (WT) and littermate POD-ATTAC mice (B). Representative Western blots of NaPi-IIa, Klotho, FRS2α, and p-FRS2α (3 animals per group) are shown. Bars (right panel) show densitometric quantification of Western blots (n=6 per group). Results are expressed as the mean ratio of individual values over the mean value obtained in control±SEM. *P<0.05.